Effect of incomplete radiofrequency ablation on the expression of MMP-9 in the rabbit VX2 model of hepatocellular carcinoma

doi:10.3877/cma.j.issn.2095-5782.2022.02.007

Abstract

Abstract:

Objective

To study the effect of incomplete radiofrequency ablation (RFA) on the expression of matrix metalloproteinase-9 protein (MMP-9) in the rabbit VX2 model of hepatocellular carcinoma.

Methods

The VX2 liver cancer model of New Zealand white rabbits was established. Thirty New Zealand white rabbits were divided into control group (n = 15) and treatment group (n = 15). The control group was only operated with switching abdomen without RFA. The treatment group was treated with RFA and the ablation range was about 75% of the tumor volume. The rapid progression subgroup after the establishment of RFA in the experimental group was defined as the tumor doubling rate at the end of the experiment was higher than that in the control group. The tumor volume and expression of MMP-9 were compared.

Results

The tumor volume was (7 862 ± 1 304) mm³ in the control group and (6 996 ± 709) mm³ in the treatment group, and the tumor multiplication rate was (291 ± 49) and (232 ± 16), respectively, the difference was statistically significant (P < 0.05). The expression rate of MMP-9 in the control gourp and the treatment group was 52.1% and 46.3 with significant differences (P < 0.05). The tumor multiplication rate and MMP-9 expression rate of rapid progression subgroup were significantly higher than that of the the treatment group, with statistically significant differences (P < 0.05).

Conclusions

Partial ablation has a certain effect on most hepatocellular carcinoma, but it may accelerate the growth of a few tumors, and the overexpression of MMP-9 may contribute to the rapid progression of residual carcinoma.

Key words: Hepatocellular carcinoma, Radiofrequency ablation, Residual carcinoma

Xiaojun Qin, Wei Wei, Zejun Yang, Manlu Wang, Guangzhi Jia, Lijun Guan. Effect of incomplete radiofrequency ablation on the expression of MMP-9 in the rabbit VX2 model of hepatocellular carcinoma[J]. Chinese Journal of Interventional Radiology(Electronic Edition), 2022, 10(02): 158-161.

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References 12

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	治疗前肿瘤体积	治疗后肿瘤体积	倍增率(%)
对照组	1 985±486	7 862±1 304	291±49
实验组	2 030±537^b	6 996±709^b	232±16
快速进展组	2 102±367^a	8 844±2 980^a	334±3^b
P值	0.338^b	0.031^b	0.029^b
P值	0.367^a	0.032^a	0.035^a

	治疗前肿瘤体积	治疗后肿瘤体积	倍增率(%)
对照组	1 985±486	7 862±1 304	291±49
实验组	2 030±537^b	6 996±709^b	232±16
快速进展组	2 102±367^a	8 844±2 980^a	334±3^b
P值	0.338^b	0.031^b	0.029^b
P值	0.367^a	0.032^a	0.035^a

	例数	MMP-9平均表达率(%)	MMP-9阳性例数占比	P值
对照组	15	52.1	12/15
实验组	15	46.3	5/15	0.025^a
快速进展组	3	74.2	3/3	0.022^b

	例数	MMP-9平均表达率(%)	MMP-9阳性例数占比	P值
对照组	15	52.1	12/15
实验组	15	46.3	5/15	0.025^a
快速进展组	3	74.2	3/3	0.022^b

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