Dasatinib-loaded macrophage membrane vesicles for the treatment of ischemic stroke

doi:10.3877/cma.j.issn.2095-5782.2022.02.005

Abstract

Abstract:

Objective

Aims to develop nanocarrier targeting the ischemic brain and the blood-brain barrier help deliver dasatinib (DAS) effectively to the brain, so as to alleviate local inflammatory response and neuronal damage after ischemic stroke (IS).

Methods

The macrophages membranes were extracted to construct DAS-loaded nanovesicles. The biosafety of DAS-loaded vesicles in IS mice was analyzed by HE staining in tissue sections of each organ. The DAS-loaded vesicles were labeled with fluorescence. Then, the brain targeting characteristic and biological distribution of DAS-loaded vesicles were analyzed by in vivo imaging and immunofluorescence. Neurological function score, mortality statistics and neuron count were performed in different treatment groups to evaluate the neuroprotective effect of DAS-loaded vesicles.

Results

DAS-loaded vesicles had good biosafety in vivo. They could rapidly and efficiently target ischemic brain tissue and be taken up by neuron. Compared with DAS, DAS-loaded vesicles significantly improved the neurological function of IS mice, reduced the mortality rate and neuronal apoptosis.

Conclusions

Our study confirmed that DAS-loaded macrophage membrane nanovesicles can safely and efficiently target the ischemic side of the brain, exert anti-inflammatory and neuroprotective effects. That provide new ideas for improving drug treatment after IS.

Key words: Ischemic stroke, Dasatinib, Macrophage membrane, Drug-loaded nanovesicles, Targeted therapy

Xiaoting Zhang, Ke Zhang, Jingpei Guo, Jiani Liu, Bin Zhou. Dasatinib-loaded macrophage membrane vesicles for the treatment of ischemic stroke[J]. Chinese Journal of Interventional Radiology(Electronic Edition), 2022, 10(02): 145-151.

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References 21

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