Application of parametric DSA color coding on skeletal muscle ischemia reperfusion injury in rabbit model

doi:10.3877/cma.j.issn.2095-5782.2018.01.016

Abstract

Abstract:

Objective:

To observe the changes of iFlow parameters of ischemia reperfusion injury in rabbit hind limb skeletal muscle after acute ischemia using parametric digital subtraction angiography (DSA) color coding (syngo iFlow) and to explore the application in evaluating the ischemia-reperfusion injury of skeletal muscle.

Methods:

Thirty New Zealand white rabbits were randomly divided into five groups, under 3 h of right hind limb ischemia followed by 0, 6, 12, and 24 h of reperfusion (IR groups, six rabbits were used for each reperfusion interval) , and six rabbits were served as Sham-operated control group. The rabbits in reperfusion groups accepted DSA scanning of double lower limbs and obtained the iFlow parameters (rPeak) by syngo iFlow software. Meanwhile, the serumal LDH, CK, MDA and SOD of the sample from jugular vein were measured, and the correlation between each iFlow parameter and serumal biochemical indicator was analyzed.

Results:

Compared with the control group, the serumal CK, LDH and MDA in IR groups increased (P<0.05) , while the value of SOD decreased with the extension duration of reperfusion (P<0.05) . Compared with the control group, the rPeak of IR groups decreased gradually with the prolongation of reperfusion (F=167.78, P<0.05) . The coefficients to correlation between rPeak and LDH, CK, MDA, SOD respectively were -0.885, -0.908, -0.541 and 0.832 (P<0.05) .

Conclusion:

The correlation between iFlow parameters and serumal biochemical indicators are fairly good, and iFlow parameters can dynamically monitor the degree of ischemia reperfusion injury skeletal muscle.

Key words: Ischemia reperfusion injury, Parametric color coding of digital subtraction angiography, Skeletal muscle

Jinwei Li, Yan Zhang, Hong Zhang, Chengzhi Li, chunxue Xu, Wentao Zhang, Wanghai Li. Application of parametric DSA color coding on skeletal muscle ischemia reperfusion injury in rabbit model[J]. Chinese Journal of Interventional Radiology(Electronic Edition), 2018, 06(01): 70-74.

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References 15

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分组	LDH(U/L)	CK(U/L)	MDA(nmol/ml)	SOD(U/ml)	rPeak
对照组	354.83±40.08	535.65±33.87	1.51±0.24	173.54±5.47	1.07±0.01
IR-0h组	495.83±57.25^a	929.17±75.18^a	1.99±0.23^a	165.79±3.78^a	0.93±0.06^a
IR-6h组	1117.33±166.91^ab	4330.22±628.87^ab	2.11±0.28^a	161.06±4.62^a	0.79±0.05^ab
IR-12h组	1574.33±238.20^abc	6636.88±395.55^abc	2.42±0.35^a	153.14±4.72^a	0.65±0.04^abc
IR-24h组	2492.33±357.32^abcd	10875.13±926.18^abcd	3.07±0.60^a	140.88±4.49^a	0.47±0.04^abcd
F值	104.54	391.54	12.37	43.34	167.78
P值	<0.05	<0.05	<0.05	<0.05	<0.05

分组	LDH(U/L)	CK(U/L)	MDA(nmol/ml)	SOD(U/ml)	rPeak
对照组	354.83±40.08	535.65±33.87	1.51±0.24	173.54±5.47	1.07±0.01
IR-0h组	495.83±57.25^a	929.17±75.18^a	1.99±0.23^a	165.79±3.78^a	0.93±0.06^a
IR-6h组	1117.33±166.91^ab	4330.22±628.87^ab	2.11±0.28^a	161.06±4.62^a	0.79±0.05^ab
IR-12h组	1574.33±238.20^abc	6636.88±395.55^abc	2.42±0.35^a	153.14±4.72^a	0.65±0.04^abc
IR-24h组	2492.33±357.32^abcd	10875.13±926.18^abcd	3.07±0.60^a	140.88±4.49^a	0.47±0.04^abcd
F值	104.54	391.54	12.37	43.34	167.78
P值	<0.05	<0.05	<0.05	<0.05	<0.05

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