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中华介入放射学电子杂志 ›› 2018, Vol. 06 ›› Issue (02) : 148 -153. doi: 10.3877/cma.j.issn.2095-5782.2018.02.012

所属专题: 文献

基础研究

大黄素甲醚调节miR-370诱导肝癌细胞凋亡的实验研究
李燕1, 潘小平1,(), 王海霞1, 仝东蒙1, 王琛1, 朱丽达1   
  1. 1. 016000 内蒙古自治区乌海市人民医院
  • 收稿日期:2017-11-29 出版日期:2018-05-01
  • 通信作者: 潘小平
  • 基金资助:
    内蒙古自治区自然科学基金项目(2017MS08166); 乌海科技计划项目(2015150305000002、2017150305000001、20171 50305000002、2017150305000003)

Regulatory effect of physcion on induced apoptosis in hepatocellular carcinoma by miR-370

Yan Li1, Xiaoping Pan1,(), Haixia Wang1, Dongmeng Tong1, Chen Wang1, Lida Zhu1   

  1. 1. The People's Hospital of Wuhai, Inner Mongolia, Wuhai 016000, China
  • Received:2017-11-29 Published:2018-05-01
  • Corresponding author: Xiaoping Pan
  • About author:
    Corresponding author: Pan Xiaoping, Email:
引用本文:

李燕, 潘小平, 王海霞, 仝东蒙, 王琛, 朱丽达. 大黄素甲醚调节miR-370诱导肝癌细胞凋亡的实验研究[J]. 中华介入放射学电子杂志, 2018, 06(02): 148-153.

Yan Li, Xiaoping Pan, Haixia Wang, Dongmeng Tong, Chen Wang, Lida Zhu. Regulatory effect of physcion on induced apoptosis in hepatocellular carcinoma by miR-370[J]. Chinese Journal of Interventional Radiology(Electronic Edition), 2018, 06(02): 148-153.

目的:

观察大黄素甲醚调节miR-370诱导肝细胞癌(HCC)细胞的凋亡情况,并探讨其作用机制。

方法:

将大黄素甲醚作用于SMMC7721和HepG2两组HCC细胞,使用TaqMan探针用实时定量PCR法检测miR-370的表达;用Western blot检测Sp1和DNMT1相应的蛋白表达水平。

结果:

大黄素甲醚抑制肝细胞癌细胞增长和诱导凋亡。肝细胞癌细胞中通过上调miR-370造成大黄素甲醚诱导型细胞凋亡。大黄素甲醚处理的细胞中通过miR-370抑制剂抑制miR-370水平能明显降低凋亡细胞的百分比(P<0.01)。大黄素甲醚通过AMPK/Sp1/DNMT1信号调节miR-370的水平(P<0.01)。AMPK/Sp1/DNMT1信号参与大黄素甲醚诱导的HCC细胞凋亡。

结论:

大黄素甲醚通过上调miR-370诱导HCC细胞凋亡,通过调节AMPK/Sp1/DNMT1信号通路对miR-370发挥调节作用。

Objective:

To observe and investigate the mechanism of induced apoptosis of hepatic cell carcinoma by miR-370.

Methods:

HCC cell lines (SMMC7721 and HepG2) were treated with physcion. MiR-370 expression was quantified by real time PCR with a TaqMan Probe and mRNA expression of Sp1 and DNMT1 was quantified by Western blot.

Results:

Physcion suppressed cell growth of HCC cells and increased the apoptotic proportion in both cell lines. Physcion induced apoptosis in HCC cells through upregulating miR-370. Our findings showed that the physcion modulated the level of miR-370 through AMPK/Sp1/DNMT1 signaling (P<0.01) .

Conclusions:

Physcion exerts anti-tumor effect against HCC, and may be a potential agent for the adjunct chemotherapy.

图1 大黄素甲醚对肝细胞癌细胞活力的影响
图2 大黄素甲醚对肝细胞癌细胞凋亡的影响(**P<0.01)
图3 HCC细胞中MiR-370介导的大黄素甲醚细胞凋亡诱导效应
图4 大黄素甲醚通过AMPK/Sp1/DNMT1信号调节miR-370的水平
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