To evaluate the pharmacokinetics and injury to eye tissue of intra-arterial-ophthalmic artery chemotherapy of carboplatin and lobaplatin in a normal porcine model.

Ten Tibetan minipigs were divided into two groups: A (carboplatin) group and B (lobetoplatin) group equally. Each pig received a single dose of either 30 mg of carboplatin (group A) or 3 mg of lobaplatin (group B) by middle meningeal artery injection into the ophthalmic artery. After the drug infusion, 2 ml of blood was taken from the femoral artery and placed in EDTA-anticoagulant purple tube at the time of 0.5 hour, 1 hour, 2 hours, 2.5 hours, 3 hours, 3.5 hours, and 4 hours after operation. The plasma is collected by centrifugation to detect the drug concentration of the sample.After the blood samples were taken, the experimental pigs were sacrificed and the bilateral eyeballs and optic nerve tissues were taken. After 10% neutral formaldehyde buffer was fixed, pathological HE staining was performed to observe the morphological changes of retinal cells and optic nerve cells.

One pig died in each group A and B, and the remaining 8 pigs completed the entire experiment. The blood drug concentrations in the carboplatin group and lobaplatin group rapidly decreased with time, and the blood concentration decreased gradually from 3 hours to 4 hours after administration. The initial phase of the drug concentration curve in the lobaplatin group was "steeper" than the carboplatin group. The retinal cell morphology was normal in both groups after ophthalmic artery instillation, and there was no cell lysis or nuclear pyknosis. The morphology of retinal arteriole endothelial cells was normal. There were no abnormalities in the morphology of the optic nerve cells in both groups.

Compared with carboplatin, the drug metabolism of lobaplatin injected through the middle meningeal artery into the ophthalmic artery of pigs was faster. There was no damage to the eye tissue and optic nerve in both groups. However, there are still deficiencies in this study, so it is still necessary to further research.