Prliminary study of effects of Ni-Ti alloy stent on the low density lipoprotein transcytosis process

doi:10.3877/cma.j.issn.2095-5782.2014.02.001

Abstract

Abstract:

Objective

To explore the effects of Ni-Ti alloy stent on the low density lipoprotein (LDL) transcytosis process in human umbilical vein endothelial cell (HUVEC) and underlying mechanism.

Methods

①Culturing the HUVEC and use transwell to establish in vitro transcytosis model, simulating the endothelial cell monolayer of blood vessels. ②Puting the Ni-Ti alloy stent on the HUVEC, adding LDL labeled by fluorescein isothiocyanate (FITC), culturing for 4 h and 24 h. ③By measuring the fluorescencent intensity of FITC in and out of the chamber to reflect the changes of transcytosis of LDL, investigating the effect of the Ni-Ti alloy stent on the LDL transcytosis process.

Results

The Ni-Ti alloy stent increase the transcytosis in 3 h and 24 h.

Conclusions

Using the trancytosis model constituted by LDL and HUVEC, Ni-Ti alloy stent can promote the trancytosis of LDL.

Key words: Low density lipoprotein, Stents, Human umbilical vein endothelial cell (HUVEC), Transcytosis

Ran Tao, Bin Xiong, Chuansheng Zheng, Ming Liang, Qi Wang, Jun Zeng. Prliminary study of effects of Ni-Ti alloy stent on the low density lipoprotein transcytosis process[J]. Chinese Journal of Interventional Radiology(Electronic Edition), 2014, 02(02): 1-3.

/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://zhjrfsxdzzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.2095-5782.2014.02.001

https://zhjrfsxdzzz.cma-cmc.com.cn/EN/Y2014/V02/I02/1

Figures/Tables 2

References 10

1	Sprague EA. In vivo cardiovascular assays for drug discovery: evolution of the drug-eluting stent[J]. Curr Opin Investig Drugs, 2007,8(3):219-225.
2	Philippe F, Dibie A, Larrazet F, et al. Drug eluting stents:from evidence based medicine to clinical practice[J]. Ann Cardiol Angiol, 2005, 54(4): 201-211.
3	Mauri L, Hsieh WH, Massaro JM, et al. Stent thrombosis in randomized clinical trials of drug-eluting stents[J]. N Engl J Med, 2007, 356(10):1020-1029.
4	Lei YP, Chen HW, Sheen LY, et al. Diallyl disulfide and diallyl trisulfide suppress oxidized LDL-induced vascular cell adhesion molecule and E-selectin expression through protein kinase A-and B-dependent signaling pathways[J]. J Nutr, 2008, 138(6):996-1003.
5	Keaney JF Jr. Atherosclerosis: from lesion formation to plaque activation and endothelial dysfunction[J]. Molecular Aspects of Medicine, 2000, 21(4-5):99-166.
6	van Beusekom HM, Whelan DM, Hofma SH, et al. Long-term endothelial dysfunction is more pronounced after stenting than after balloon angioplasty in porcine coronary arteries[J]. J Am Coll Cardiol, 1998, 32(4):1109-1117.
7	Bian F, Yang X, Zhou F, et al. CRP promotes atherosclerosis by increasing LDL transcytosis across endothelial cells[J]. Br J Pharmacol, 2014, 171(10):2671-2684.
8	Biffl W.L, Moore EE, Moore FA, et al. Nitric oxide reduces endothelial expression of intercellular adhesion molecule (ICAM)-1[J]. J Surg Res, 1996,63(1):328-32.
9	Chandrasekar B, Mummidis S, Mahimainathan L, et al. Interleukin-18-included human coronary artery smooth muscle cell migration is dependent on NF-kappaB-and AP-1-mediated matrix metalloproteinase-9 expression and is inhibited by atorvastatin[J]. J Biol Chem, 2006, 281(22):15099-15109.
10	Goldschmidt-Clermont PJ, Greager MA, Losordo DW et a1. Atherosclerosis 2005 Recent Discoveries and Novel Hypotheses[J]. Circulation, 2005, 112(21):3348-3353.

[1]	Yi Li, Hongjin Wang, Yanping Feng, Kewei Zhang, Xiaowei Wu. Study on the construction of the genipin-chitosan collagen scaffold for yak achilles tendon and its cytotoxicity and histocompatibility [J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2019, 14(06): 410-415.
[2]	Jianting Gao, Jianbiao Lin, Guojun Liu, Cong Zhu, Benwen Wu, Zhenqi Ding, Guofeng Huang. Stromal cell-derived factor-1 α combined with Integra scaffold promotes healing of full-thickness skin defects [J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2019, 14(05): 355-360.
[3]	Zhiyong Mei, Qing Pang, Yong Wang, Zhongran Man, Hao Jin, Huichun Liu. Comparison of the efficacy between stent combined with ¹²⁵I seeds intracavitary irradiation and palliative surgery in advanced extrahepatic cholangiocarcinoma [J]. Chinese Archives of General Surgery(Electronic Edition), 2021, 15(06): 412-417.
[4]	Youjun Mao, Xiaoqiang Li. Diagnosis and treatment of in-stent restenosis of lower extremity artery [J]. Chinese Archives of General Surgery(Electronic Edition), 2020, 14(01): 76-80.
[5]	Haixiong Zhang, Huanwei Chen. Comparative study of different bile duct closure methods for laparoscopic common bile duct discovery for choledocholithiasis [J]. Chinese Archives of General Surgery(Electronic Edition), 2019, 13(03): 208-212.
[6]	Sijun Cheng, Yumei Yang, Bo Yuan, Jianli Lu, Chao Deng. Selective left hemicolectomy after expandable stent placement to relieve obstruction combined with neoadjuvant chemotherapy in the treatment of obstructive left colon cancer [J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2021, 15(04): 430-433.
[7]	Zhaohui Zeng, Xiaoqing Zheng, Minbo Yan, Hao Zheng, Shuchang Huang, Xi Chu, Zhenyu Guo. Non-cystoscopic removal of retained ureteral sents: What we need is just a segment of Vicryl Rapide and a silk thread [J]. Chinese Journal of Endourology(Electronic Edition), 2020, 14(03): 189-191.
[8]	Jie Liu, Jianghui Xue, Guangyong Ran, Xuehui Hu, Wei Wang. Treatment of ureterostenosis secondary to ureteral calculi with two ipsilateral double J stents [J]. Chinese Journal of Endourology(Electronic Edition), 2019, 13(04): 251-254.
[9]	Zhaohui Tang, Jiansheng Zhang, Qilong Chen, Weiwei Deng, Jin Wang. Optimal single-energy image of spectral CT in stent evaluation after TIPS [J]. Chinese Journal of Hepatic Surgery(Electronic Edition), 2022, 11(05): 463-468.
[10]	Xiaoyu Fan, Sizheng Sun, Yimin E, Chunzhao Yu. Different surgical options for obstructive left colon cancer [J]. Chinese Journal of Colorectal Diseases(Electronic Edition), 2023, 12(06): 500-504.
[11]	Yueyan Xiao, Wenbin Wei, Ping Xi, Bo Gao, Jianan Wang, Yunyun Geng, Min Zheng, Fengying Jiao. Epidemiological analysis on the systemic risk factors for the idiopathic macular membrane in the community residents over 60 years old [J]. Chinese Journal of Ophthalmologic Medicine(Electronic Edition), 2022, 12(05): 281-286.
[12]	Zhongmei Ren, Anxin Zhang, Chunli Xing. Relationship between serum factor, pulmonary function and obstructive sleep apnea syndrome in patients with acute ischemic stroke [J]. Chinese Journal of Brain Diseases and Rehabilitation(Electronic Edition), 2019, 09(03): 137-141.
[13]	Jianjian Chen, Yuan Yao, Xueliang Zhou, Dechao Jiao, Xinwei Han. Current status and research progress of metal stent combined with intraluminal antineoplastic technique in treatment of malignant biliary obstruction [J]. Chinese Journal of Interventional Radiology(Electronic Edition), 2019, 07(02): 163-167.
[14]	Haijing Zhang, Haiqing Hu. Clinical progress on treatment of benign stenosis of esophageal anastomosis [J]. Chinese Journal of Gastrointestinal Endoscopy(Electronic Edition), 2019, 06(01): 27-31.
[15]	Zhiyong Tong, Yuan Liu, Qichen Pan, Cunyi Zou, Deshu Cheng, Xudong Zhao, Jinsong Zhang, Youhe Jin, Zhe Lou. Long-term outcomes of carotid endarterectomy with patch angioplasty in the treatment of in-stent restenosis [J]. Chinese Journal of Cerebrovascular Diseases(Electronic Edition), 2022, 16(06): 398-407.

Please choose a citation manager

Content to export