As an emerging interventional ablation modality which is different from conventional physical ablation such as radiofrequency ablation, microwave ablation and cryoablation, irreversible electroporation(IRE) ablationutilizes high voltage pulses to create permanent nanopores in the cell membrane, which in turn induces apoptosis of targeted cells. The promising technology can be used to ablate tumors adjacent to vital structures due to its less impact on extracellular matrix. Pancreatic cancer, despite extensive research, remains the most aggressive gastrointestinal tumors, having a poor prognosis and ambiguous early symptom usually diagnosed at stage Ⅲ or Ⅳ. Recently, IRE ablation has being progressively applied in the management of unresectable pancreatic cancer by virtue of its high efficiency and safety. This article will review on the basic research of IRE, clinical application in pancreatic cancer and some indefinite questions.